Successful transplantation of spermatogonial stem cells into the seminiferous tubules of busulfan-treated mice

Abstract Background Spermatogonial stem cells (SSCs) in the testis are crucial for transferring genetic information to next generation. Successful transplantation of SSCs infertile men is an advanced therapeutic application reproductive biology research. Methods In this experimental research, both vitro and vivo characterization undifferentiated differentiated were performed by morphology—immunocytochemistry (ICC), immunohistochemistry (IMH), Fluidigm Real-Time polymerase chain reaction (RT-PCR) flow cytometry analysis. The isolated finally microinjected into rete busulfan-treated mice. compact more loosely connected round during testicular cell expansion from their specific feeder layer. Results ICC analysis indicated high low expression levels Zbtb16 germ cells. Also, IMH showed different two populations tissue. While RT-PCR overexpression TAF4B gene, DAZL, VASA, down-regulated differentiation ( P < 0.05). confirmed significant downregulation Itgb1 Itga4 differentiation. By NOD/SCID mice, GFP-labeled sperm developed. Conclusions current study, we a technique that could be useful future microinjection infertility treatment studying sperm.